Gene Description

Precision Medicine Experts at RPRDx

Gene Description

CACNA1S

The CACNA1S gene encodes the α 1s sub-unit of the dihydropyridine receptor (DHPR), a voltage-gated calcium channel. RYR1 and CACNA1S are loci for the pharmacogenetic trait of malignant hyperthermia (MH), a condition that is predisposition to a hypermetabolic reaction triggered by the potent volatile anesthetics or the depolarizing muscle relaxant succinylcholine.1,2  MH susceptibility (MHS) is inherited in an autosomal dominant pattern, and a heterozygous genotype of a pathogenic variant in RYR1 and CACNA1S can be considered as diagnostic for the trai.3

The diagnosis of MH susceptibility (MHS) is made by one of two criteria: (i) positive response to an in vitro muscle bioassay, such as the in vitro contracture test (IVCT), or the caffeine- halothane contracture test (CHCT), as it is known in the United States; or (ii) the presence of a pathogenic variant in RYR1 or CACNA1S found by molecular genetic testing.4 Both the CHCT and IVCT can be difficult to perform, as it requires a muscle biopsy at a specialized MH biopsy testing center. And there are only four testing centers in the United States.5 Both the Malignant Hyperthermia Association of the United States (MHAUS) and the European Malignant Hyperthermia Group (EMHG) consider the genetic testing of RYR1 and CACNA1S to be a viable diagnostic approach for MHS.

The latest Clinical Pharmacogenetics Implementation Consortium (CPIC) guideline recommends only non-triggering anesthetic agents for a person with MHS. 4

It is worth noting that the American College of Medical Genetics and Genomics has included RYR1 and CACNA1S in its list of genes for which pathogenic variants should be returned as secondary findings. RPRD’s WPS testing only screens for specific known variants associated with PGx function and a negative/normal test result does not rule out the presence of other possible pathogenic variants in these genes.

Click here for the full CPIC Guideline for the use of potent volatile anesthetic agents and succinylcholine in the context of CACNA1S or RYR1 genotypes.

References
  1. Alvarellos ML, et al. PharmGKB Summary: Very Important Pharmacogene Information for CYCNA1S. 2018.
  2. Hopkins PM. Malignant Hyperthermia: Pharmacology of Triggering. Br J Anaesth. 2011;107:48-56.
  3. Rosenberg H, et al. Malignant Hyperthermia: A Review. Orphanet. J Rare Dis. 2015;10:93.
  4. Gonsalves SG, et al. Clinical Pharmacogenetics Implementation Consortium (CPIC) Guideline for the Use of Potent Volatile Anesthetic Agents and Succinylcholine in the Context of RYR1 or CACNA1S Genotypes. Clin Pharmacol Ther. 2018 Nov [Epub ahead of print]
  5. https://www.mhaus.org/testing/muscle-biopsy-chct/chct-muscle-biopsy-testing-centers/